The purpose of hybridoma technique is the production of homogeneous antibody against a specific epitope of an antigen in vitro. These monoclonal antibodies have wide applications such as:
? Immunodiagnostic reagents, either for detection of the disease causing agents directly in tissues or body fluids or used in indirect diagnosis like serological detection of antibodies to the antigenic agent.
? Hybridoma technology is useful by producing broad spectrum of monoclonal antibody which are useful in cancer therapy, bone marrow transplantation, treating cardiovascular diseases like Myocardial infarction by Myoscint, Deep vein thrombosis by 99mTc-Fab fragment, Atherosclerosis etc.
? Hybridoma is now a hot cake technology for diagnosis of autoimmune diseases.
? For Biotechnological application the MoAbs is used for purification of protein, antigens, Drugs treating and Gene cloning & expression.
? It is a research tool for the recognition of specific amino acid sequences of polypeptides.